HLA-B * 1502 Gene Detection Kit (Fluorescent Probe qPCR Method)

Detection principle:
Design specific primers and probes based on the known gene sequence of human major histocompatibility antigen molecules. When the primer sequence can fully match the target sequence, the primers undergo polymerase chain reaction. Special nucleic acid fragments will be replicated and amplified, while the probe will bind to the target sequence and generate fluorescence through the decomposition of polymerase. Fluorescence reaction indicates the presence of a target gene sequence that matches specific primers in the sample, otherwise it is not.

  

Detection significance:
The HLA-B * 1502 allele has been confirmed to be highly associated with Steven Johnson Syndrome (SJS) caused by the use of the commonly used psychiatric drug carbamazepine in the Han population, as well as toxic epidermal necrolysis (TEN) with a clinical mortality rate of up to 30%. Research has found that patients with HLA-B * 1502 gene who take this medication have a 193-1300 times higher risk of severe drug allergies causing these two symptoms compared to the general population. According to reliable statistics, the proportion of carrying HLA-B * 1502 allele in the Chinese population exceeds 10%. Therefore, by detecting whether the HLA-B * 1502 allele is carried, the risk of patients taking carbamazepine induced SJS/TEN can be reduced. (The US FDA and medical regulatory authorities in Japan, Taiwan, and other regions have explicitly required doctors to undergo HLA-B * 1502 genetic testing before using carbamazepine in Asian populations, and this test has been included in medical insurance coverage.)

Product features:
Specific: Based on the fluorescent probe PCR method, the specificity is higher than that of ordinary PCR and fluorescent dye PCR methods
Accuracy: Amplify using three reaction wells, each containing specific primers and probes as well as internal control primers and probes, to maximize the differentiation of HLA-B * 1502 type
Convenient: Easy to operate, no need for electrophoresis
Time saving: The detection time is short, about one hour to complete the detection
Reliable: Quality control passed, results are reliable
Anti pollution: No need for electrophoresis to prevent contamination during the electrophoresis process

Detection process:

  

Result interpretation:
Each sample needs to undergo three tube reactions simultaneously: MIX1, MIX2, and MIX3.

Note: "/" indicates not considering
Note 1: Indicators as B * 1502, B * 1588, B * 1589, or B * 9521
Note 2: If the same result is still obtained, it will be judged as negative
Ordering information:

Contact information:
Address: 225300, East Side, 2nd Floor, G75, Phase 4, Yaocheng, Taizhou Pharmaceutical High tech Zone, Jiangsu Province
Phone: 0523-86201335 Extension product consultation: 8010
Fax: 0523-86201325
Website: www.wehelpinc.com
Email: sales@wehlepinc.com (Product Consultation)

This product is for scientific research purposes only

Previous Product： CYP3A5 (fluorescent probe qPCR method)

Next Product： HLA-B * 5801 Gene Detection Kit (Fluorescent Probe qPCR Method)