CYP3A4 genotyping products

[Product Name] Common Name: Cytochrome P450 3A4 Genotyping Detection Reagent Disk (Fluorescence PCR Method)

[Intended use] This reagent kit is used for typing and qualitative detection of cytochrome P450 3A4 gene subtypes, including CYP3A4 * 1 subtype and CYP3A4 * 18B subtype Cytochrome P450 (CYP) is an important drug metabolism enzyme in the human body. CYP3A is an enzyme in the CYP superfamily, highly expressed in the liver and intestines, and CYP3A4 and CYP3A5 are important subtypes of this enzyme. At present, research has found that CYP3A4 and CYP3AS jointly participate in the metabolism of immunosuppressive agents cyclosporine A (CsA) and tacrolimus (FK506), and their activity is related to the blood drug concentrations of CsA and FK506. The CYP3A4 gene is located on human chromosome 7 q211-22.1, with a total length of 272kb, including 13 exons and encoding 502 amino acids. The 8% difference in CYP3A4 activity between individuals is determined by genetic factors. More than 40 alleles of CYP3A4 have been identified, and the frequency of single nucleotide polymorphism (SNP) loci in CYP3A4 varies among different races.

[Testing principle] This reagent uses real-time fluorescence PCR combined with Toqman probe technology to qualitatively genotype the CYP3A4 gene. Based on the CYP3A4 gene sequence published in GENBANK data, specific primers were designed using the amplification inhibition mutation system (ARMS) analysis method. The specific probes were labeled with FAM, and two tube reactions were performed for each detection to detect the CYP3A4 subtype. At the same time, a pair of internal standard primers and probes were added to the tube, labeled with HEX, to monitor false negative results caused by instrument failures, reagent factors, polymerase activity, or inhibitors in the sample. The probe is an oligonucleotide consisting of a 3 'end reporter group and a 3' end quenched group. During PCR amplification, when the probe is intact, the fluorescence emitted by the quenched group is absorbed by the quenched group when the quenched group is close to the reporter group, and no fluorescence signal is emitted. When the primer is extended, the probe bound to the template is cut off by Taq enzyme (5 ° → 3 ° exonuclease activity), and the reporter group is separated from the quenched group to generate a fluorescence signal. The fluorescence quantitative PCR instrument automatically draws a time protection curve based on the detected fluorescence signal. Thus achieving qualitative typing detection of CYP3A4 gene

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