HLA-B * 27 Gene Detection Kit (Fluorescent Probe qPCR Method)

[Detection principle]
This reagent kit uses real-time fluorescence PCR combined with Taqman probe technology. A specific primer probe is designed based on the known HLA-B * 27 gene sequence. The probe is composed of oligonucleotides including a 5 'end reporter gene and a 3' end quenched group. During PCR amplification, when the probe is intact, the quenched group approaches the reporter gene, and the fluorescence emitted by the reporter gene is absorbed by it without emitting a fluorescence signal. The fluorescence quantitative PCR instrument automatically draws a real-time amplification curve based on the detected fluorescence signal, thereby achieving qualitative analysis of the target gene.
This product has designed a set of B * 27 gene specific primer probes, labeled with FAM, and can detect the B * 27 gene by performing one tube reaction each time. In addition, HEX is used as an internal reference in each reaction to detect whether the various factors in the reaction system are normal.

[Detection significance]
The Human Leukocyte Antigen B * 27 Nucleic Acid Detection Kit (Fluorescence PCR Method) uses Taqman probe technology to design specific primers and probes, detects target genes using a fluorescence PCR instrument, and determines whether it is a B * 27 gene based on the curve drawn from the final fluorescence signal.
Human leukocyte antigen molecules play an extremely important role in the specific identification of T cell immunity. HLA-B * 27 is one of the HLA-B site types, and its association with ankylosing spondylitis has been widely studied and proven. Ankylosing spondylitis is a condition of the spine and
Chronic inflammatory response of the interosseous joint of the corpus callosum. The vast majority of patients with ankylosing spondylitis have positive HLA-B * 27 results, so HLA-B * 27 is often used as a reference for the diagnosis of mandatory spondylitis in clinical practice.


[Product features]
Specific: Based on the fluorescent probe PCR method, the specificity is higher than that of ordinary PCR and fluorescent dye PCR methods;

Convenience: By detecting one tube of reaction each time, the B * 27 gene can be detected. The operation is simple and does not require electrophoresis;

Reliable: Quality control passed, results are reliable; Anti pollution, no need for electrophoresis, avoiding uncontrollable pollution caused by electrophoresis and other factors;

Registration certificate number: National Machinery Registration No. 20233400489

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