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Based on the previous understanding, the human MHC gene, also known as HLA, encodes molecules that are expressed on different cell surfaces, participate in antigen presentation, constrain intercellular recognition, and induce immune responses. It is known that HLA plays an extremely important role in the specific identification of T cell immunity and the medication judgment of some clinical diseases.
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The necessity of detecting HLA-B * 5801
HLA-B * 5801 is associated with allergic reactions to the commonly used drug allopurinol, which is used to treat gout and hyperuricemia. Studies have found that individuals with the genetic marker HLA-B * 5801 have a significantly increased risk of blindness, mortality, and Steven Johnson Syndrome (SJS) when taking this drug.
Our company's human major tissue compatibility antigen molecular specific primer fragment B * 5801 fluorescence typing kit uses sequence specific primers for fluorescence polymerase chain reaction to identify the molecular types of human major tissue compatibility antigens.
HLA-B * 5801 Fluorescence Typing Kit
The US FDA and medical regulatory authorities in Japan, Taiwan, and other regions have explicitly required doctors to undergo HLA-B * 5801 genetic testing before using Allopurinol on Asian populations, and this test is included in medical insurance coverage.
Detection principle
Identification of molecular types of human major histocompatibility antigens using sequence specific primers using fluorescent polymerase chain reaction.
Firstly, specific primers and probes are designed based on the known gene sequence of human major histocompatibility antigen molecules. When the primer sequence can fully match the target sequence, polymerase chain reaction is initiated. Special nucleic acid fragments will be replicated and amplified, while the probe bound test sequence will be quenched by polymerase cleavage activity to produce fluorescence.
Schematic diagram of detection principle
Fluorescence reaction indicates the presence of a target gene sequence that matches specific primers in the sample, otherwise it is not. The detection system design has internal controls.
Kit specifications
There are a total of 3 types of reaction tubes (MIX1, MIX2, MIX3);
The MIX-1, MIX-2, and MIX-3 reaction tubes contain nucleic acid primers and probes required for measuring B * 5801;
Main reaction solution, 220 per tube μ L;
Positive control, 50 per tube μ L;
Product features
The method is specific, accurate in detection, easy to operate, and the results are reliable.
Interpretation of test results
According to the result interpretation table in the product manual, it is possible to clearly interpret the positive/negative situation of HLA-B * 5801 test sample.
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